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Guide for Column Selection to Achieve Productivity and Analysis Results

Guide for Column Selection to Achieve Productivity and Analysis Results

We deliver innovative columns supported by experience, achievements, and high technology and quality!

This document explains the selection of columns that achieve productivity and analytical results. It includes an introduction to Agilent J&W GC columns, principles of column selection, and details on stationary phase selection. The Ultra Inert columns have set the industry standard due to their inertness and low column bleed, allowing for lower detection limits even for difficult-to-analyze compounds and enabling accurate data acquisition. 【Contents (Excerpt)】 ■ Introduction to Agilent J&W GC columns ■ Principles of column selection ■ Selection of stationary phases ■ Column inner diameter ■ Column length *For more details, please refer to the PDF document or feel free to contact us.

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Low bleed GC/MS column "VF-1ms"

Low bleed GC/MS column "VF-1ms"

High precision and high sensitivity non-polar columns! Measuring with good sensitivity across a wide range of application fields.

We would like to introduce the 'VF-1ms' that we handle. It is a non-polar, ultra-low bleed GC column that has undergone advanced deactivation treatment to achieve improved sensitivity in various analyses. Using 100% dimethylpolysiloxane as the stationary phase, it guarantees a bleed value of less than 1 pA at 325°C (for 0.25mm × 30m, 0.25μm). 【Features】 ■ Guarantees ultra-low bleed suitable for trace level analysis ■ Applicable to a wide range of analyses ■ Provides high-precision analytical results with advanced deactivation treatment ■ Maximum operating temperature: 325°C (isothermal), 350°C (temperature ramp) ■ Minimum operating temperature: -60°C *For more details, please refer to the PDF document or feel free to contact us.

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ZORBAX Bonus-RP

ZORBAX Bonus-RP

Introducing a new technology that enables three-dimensional protection! ZORBAX reverse phase HPLC columns.

The "ZORBAX Bonus-RP" has a unique structure that incorporates polar amide groups into a long alkyl chain. This new bonding method suppresses the interaction between basic compounds and the silica substrate, significantly improving peak shape even for particularly difficult-to-separate basic compounds. It also demonstrates excellent stability at pH 2, making it suitable for improving the peak shape of basic compounds with significant tailing in many other alkyl-type stationary phases. 【Features】 ■ Excellent peak shape for challenging basic compounds at low and neutral pH ■ Unique selectivity among reverse phase columns ■ New technology that embeds polar groups and allows for steric protection ■ Usable with 100% aqueous mobile phases *For more details, please refer to the PDF document or feel free to contact us.

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Product Catalog: AGILENT INFINITYLAB

Product Catalog: AGILENT INFINITYLAB

Introducing a variety of products! Solutions to enhance the workflow of liquid chromatography.

This catalog introduces the "INFINITYLAB LC Series," designed to integrate LC instruments, columns, and consumables for seamless operation. We offer a lineup that provides the highest level of efficiency, including the "INFINITYLAB LC Columns," which deliver stable throughput and quality at a high standard, as well as the "INFINITYLAB LC Series." Additionally, we feature "INFINITYLAB LC Consumables" that make daily tasks more efficient. Please use this information when selecting products. [Contents] ■ INFINITYLAB LC Series ■ INFINITYLAB LC Columns ■ INFINITYLAB LC Consumables *For more details, please refer to the PDF document or feel free to contact us.

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ZORBAX Carbohidrate Analysis

ZORBAX Carbohidrate Analysis

It can handle a large injection volume of 50μL with a 4.6×150mm column!

The "ZORBAX Carbohydrate Analysis" is a column that excels in reproducibility, efficiency, and flexibility. These columns utilize ZORBAX porous silica fine particle technology. The silica is manufactured, bonded, and packed at Agilent facilities certified with ISO 9001. 【Features】 ■ Reproducibility ■ Efficiency: Utilizes ZORBAX porous silica fine particle technology ■ Flexibility: Capable of handling large volume injections of 50μL with a 4.6×150mm column ■ Recommended for use with a differential refractive index detector (RID) *For more details, please refer to the related links or feel free to contact us.

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ProSEC 300S

ProSEC 300S

The filler has a nominal pore size of 300 Å! It is suitable for the analysis of a wide range of spherical proteins.

The "ProSEC 300S" is a silica-based column specifically designed for analyzing proteins using size exclusion chromatography. By removing acidic silanol and reducing interaction effects through a unique surface modification, it minimizes non-size exclusion effects across various types of proteins. With a particle size of 5μm, it offers high efficiency and improved separation capability, making it suitable for all protein analyses using salt buffer solutions. 【Features】 ■ Uses robust silica particles with no bleed during use ■ A single column with a wide linear separation range ■ Column dimensions compatible with multi-detector systems *For more details, please refer to the related links or feel free to contact us.

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PL-SAX and PL-WAX for biomolecules

PL-SAX and PL-WAX for biomolecules

LC columns for biopharmaceutical analysis! Polymers are macro-porous spherical styrene-divinylbenzene.

We would like to introduce our products, "PL-SAX & PL-WAX for Biomolecules." "PL-SAX" is a column used in hydrophilic strong anion exchange chromatography. Additionally, due to its high physical stability, it can also be applied for high-speed separations using high flow rates and rapid gradients. Thanks to this excellent stability, the column can be equilibrated quickly, and it is also possible to clean the column with organic solvents, strong acids/strong bases, and high salt concentration solutions. 【Features (Specifications)】 ■ Polymer: Macroporous spherical styrene-divinylbenzene ■ Particle sizes: 5μm, 8μm, 10μm, 30μm ■ Pore sizes: 1000Å, 4000Å ■ Bonding functional groups: Trimethylamino groups ■ Concentration of organic solvents used: 0 to 100 *For more details, please refer to the related links or feel free to contact us.

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PLRP-S for bioanalysis

PLRP-S for bioanalysis

We offer a wide range of pore sizes and particle sizes! All have the same chemistry and fundamental adsorption characteristics.

We would like to introduce our LC column for the analysis of biopharmaceuticals, the 'PLRP-S for Bioanalysis'. Due to the hydrophobic nature of the polymer particles, there is no need for a bonded phase or alkyl bonding groups for separation in reverse phase systems. Therefore, residual silanol and heavy metal contaminants are absent, which does not affect the analysis results. We have suitable columns for separation and preparative purification for analytical purposes, and we can also provide process columns filled with bulk packing materials. 【Features】 ■ Thermally and chemically stable ■ Compliant with USPL21 ■ Pore sizes (100Å to 4000Å) suitable for separating complexes from low to high molecular weights and polynucleotides ■ Contains durable and robust polymer particles that provide highly reproducible results and a long column lifespan *For more details, please refer to the related links or feel free to contact us.

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DB-FATWAX Ultra Inert

DB-FATWAX Ultra Inert

Achieving high inactivity with difficult-to-separate fatty acids, resulting in improved selectivity of fatty acid methyl esters!

The "DB-FATWAX Ultrapure" is a WAX-type GC column specialized for applications. It is well-suited for the analysis of methyl esters of unsaturated/polyunsaturated fatty acids found in fish oil and animal fats. Thanks to Agilent's unique Ultrapure technology, which offers excellent inertness and thermal stability, it is also suitable for the analysis of challenging non-derivatized fatty acids. 【Features】 ■ Individually tested with FAME mixtures ■ Reproducible FAME equivalent chain length values ■ Highly reliable inert performance between columns ■ Improved peak shape for challenging polar compounds such as free fatty acids ■ High polarity, equivalent to USPG16 ■ The chemical bonding and cross-linked stationary phase of the column can be cleaned with solvents ■ Compatible with the injection of water-soluble samples *For more details, please refer to the PDF document or feel free to contact us.

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GC parts and consumables: GS-OxyPLOT

GC parts and consumables: GS-OxyPLOT

A stable phase that eliminates particle generation and detector spikes! Suitable for low-concentration quantitative GC analysis.

"GS-OxyPLOT" is a suitable product for ASTM methods for oxygenated compounds. It can also be used instead of GC columns with gas-liquid phases such as TCEP for similar applications. The maximum temperature is 350°C, and there is no bleed typically seen in liquid and siloxane-coated GC columns. 【Features】 ■ Precise analysis of oxygenated compounds at ppm/ppb concentrations in C1 to C10 hydrocarbons ■ High selectivity for a wide range of oxygenated compounds (ethers, alcohols, aldehydes, ketones) in complex matrices such as gaseous hydrocarbons, automotive fuels, and crude oil ■ Suitable for ASTM methods for oxygenated compounds ■ Extremely high column stability with no column bleed (maximum temperature 350°C) ■ Stable phase that eliminates particle generation and detector spikes ■ Suitable for low-concentration quantitative GC analysis *For more details, please refer to the PDF document or feel free to contact us.

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Column pair 'DB-CLP1/CLP2'

Column pair 'DB-CLP1/CLP2'

Eliminate the need for column exchange! The medium-polarity stable phase enables reliable analysis with high speed and low bleed.

The "DB-CLP1/CLP2" is a highly flexible universal column pair for nine types of EPA dual ECD pesticide methods. The medium-polarity stable phase enables reliable analysis with high speed and low bleed. The reliable high-speed column provides excellent separation capability along with outstandingly low bleed, eliminating the need for time-consuming column exchanges. 【Features】 ■ Universal column pair designed for pesticide analysis ■ Suitable for dual column, dual ECD GC analysis ■ Medium-polarity stable phase enables reliable analysis with high speed and low bleed ■ Eliminates the need for time-consuming column exchanges ■ Tested with strict testing procedures, with performance summary sheets attached to all columns *For more details, please refer to the PDF materials or feel free to contact us.

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ZORBAX Eclipse XDB

ZORBAX Eclipse XDB

An ideal choice for method analysis! Long lifespan at neutral pH due to high-density chemical bonding and double-end capping.

The "ZORBAX Eclipse XDB column" is designed to separate basic, acidic, and neutral compounds with good peak shape over a pH range of 2 to 9. It features a fully porous spherical packing material with a particle size of 1.8 μm suitable for Rapid Resolution High Throughput (RRHT). The Eclipse XDB-C18 and C8 provide high separation efficiency and long column life in the neutral range. Additionally, method optimization can be achieved by changing the mobile phase, pH, column temperature (up to 60°C), or by switching the stationary phase to Eclipse XDB-Phenyl. 【Features】 ■ Excellent peak shape for bases, acids, and neutral substances ■ Fully porous spherical packing material with a particle size of 1.8 μm suitable for Rapid Resolution High Throughput (RRHT) ■ Expanded pH range from 2 to 9 ■ Long lifespan at neutral pH due to high-density chemical bonding and double-end capping *For more details, please refer to the PDF document or feel free to contact us.

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ZORBAX Eclipse XDB-CN

ZORBAX Eclipse XDB-CN

Introducing the ZORBAX normal phase HPLC columns with both combined and non-combined filler series!

We would like to introduce the 'ZORBAX Eclipse XDB-CN' that we handle. We offer both bonded and non-bonded filler series, which are suitable for the normal phase analysis of basic compounds. It equilibrates faster than ZORBAX Rx-Sil and can be used for many normal phase analyses similar to Rx-Sil. 【Features】 ■ Manufactured from high-purity Rx-SIL ■ Equilibrates faster than ZORBAX Rx-Sil and can be used for many normal phase analyses similar to Rx-Sil *For more details, please refer to the PDF document or feel free to contact us.

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ZORBAX Bio-SCX Series ii

ZORBAX Bio-SCX Series ii

LC and LC/MS columns! It is possible to achieve high retention and excellent peak shape.

The "ZORBAX Bio-SCX Series II" is a column designed for optimal 2D separation of peptides and proteins using LC/MS. The packing is based on 3.5μm ultra-high purity ZORBAX silica particles, combined with a polymer that is functionalized with sulfonic acid groups, taking into account environmental considerations. As a result, high retention and excellent peak shape can be achieved in the ion exchange procedures for 2D analysis of peptides and proteins. 【Features】 ■ Available in column configurations of 35mm length × 0.3mm inner diameter and 50mm length × 0.8mm inner diameter ■ pH range: 2.5 to 8.5 *For more details, please refer to the related links or feel free to contact us.

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Bond Elut C1

Bond Elut C1

Bond Elut C1 has low retention capacity for all alkyl group bonding phases of nonpolar compounds because it is a single carbon functional group.

Bond Elut C1 is a single carbon functional group, resulting in low retention capacity for all alkyl group bonds in non-polar compounds. However, due to the end-capping, the silanol activity is suppressed. As a result, the retention and elution of polar and multifunctional compounds become easier. *For more details, please refer to the PDF document or feel free to contact us.*

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